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Jackson Immuno alexa fluor 647 affinipuretm goat anti rabbit igg h l
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Signal Pharmaceuticals pil-6(2225/111)-luc containing nucleotides 2225/111 of the human il-6 promoter
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Jackson Immuno goat antirabbit cy3
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
Goat Antirabbit Cy3, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology puri ed mouse monoclonal antibody
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
Puri Ed Mouse Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno colloidal gold conjugated goat anti rabbit igg
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
Colloidal Gold Conjugated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno horseradish peroxidase conjugated goat anti rabbit
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
Horseradish Peroxidase Conjugated Goat Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carna Inc dlk(map3k12)
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
Dlk(map3k12), supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ESHA Research food processor v. 11.1
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
Food Processor V. 11.1, supplied by ESHA Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cook MyoSite Inc primary human skeletal derived myoblasts
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
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Innovotech inc 96-well microtiter plates with peg lids
FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and <t>GAM-Cy3</t> (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.
96 Well Microtiter Plates With Peg Lids, supplied by Innovotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and GAM-Cy3 (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.

Journal: Journal of neurochemistry

Article Title: Caffeine stimulates amyloid beta-peptide release from beta-amyloid precursor protein-transfected HEK293 cells.

doi: 10.1046/j.1471-4159.1997.69041580.x

Figure Lengend Snippet: FIG. 4. Immunocytochemical dem- onstration of ßAPP and RYR5 in HEK293ßApp75l cells. A and B, D and E, and G and H are double-immu- nofluorescence photomicrographs. A: RYR localization, using anti- bodies XA7 (1:20) and GAM-Cy3 (1:250). B: The same field as in A, stained with antibodies aB5 (1:200) and GAR-Cy2 (1:250). ßAPP accumulations are similar, but not identical, to RYR localiza- tion (arrows). C: A magnified area of cytoplasmic processes stained with anti-XA7 showing a radial-lin- ear pattern (arrows). D: Eccentric accumulations of /3APP (anti-aB5) colocalize with a Golgi marker (arrows) (anti-58K, 1:50) in E. F: Cellular ßAPP labeled with anti- 5a3/1G7. G: /3APP immunostain- ing pattern (aB5) is compared with the ER marker anti-PDI (1:200) in H. I: The ßAPP (aB5) pattern is re- distributed in brefeldin-pretreated cells. J and K: Cell surface /3APP was labeled with aB5 at 4°Cand internalized at 25°Cfor 7.5 min in J and 20 min in K. Bars = 10 pm.

Article Snippet: Conjugated secondary antibodies used for immunocytochemistry were 1:250 dilutions of goat anti-mouse CY3, biotinylated goat anti-rabbit, goat antirabbit CY3 (Jackson ImmunoResearch), and goat antimouse CY2, streptavidin CY2 (Biological Detection Systems).

Techniques: Staining, Marker, Labeling